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The objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mastitis; Sheep; Staphylococcus aureus; Antibiotic resistance; Virulence factors. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200535 Community-acquired methicillin-resistant Staphylococcus aureus from skin and soft tissue infections (in a sample of Egyptian population): analysis of mec gene and staphylococcal cassette chromosome BJID Sobhy,Nagat; Aly,Fatma; El Kader,Ola Abd; Ghazal,Abeer; Elbaradei,Amira. BACKGROUND: Staphylococcus aureus has been recognized as an important pathogen associated with inpatients and community infections. Community-acquired methicillin-resistant S. aureus (CA-MRSA) infections commonly present as skin and soft-tissue infections (SSTIs). Treatment often includes incision and drainage with or without adjunctive antibiotics. OBJECTIVES: This study aimed to identify CA-MRSA infections both phenotypically and genotypically, to determine their spectrum of antibiotic resistance, and to establish the best scheme for molecular distinction between hospital-acquired MRSA (HA-MRSA) and CA-MRSA by staphylococcal cassette chromosome mec (SCCmec) typing and detection of Panton Valentine leukocidin (PVL). MATERIALS: 50 swabs, from skin and soft... Tipo: Info:eu-repo/semantics/article Palavras-chave: Community-acquired; Methicillin-resistant; Staphylococcus aureus. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000500004 Community-associated methicillin-resistant Staphylococcus aureus in non-outbreak skin infections BJM Bonesso,Mariana Fávero; Marques,Silvio Alencar; Camargo,Carlos Henrique; Fortaleza,Carlos Magno Castelo Branco; Cunha,Maria de Lourdes Ribeiro de Souza da. The aim of this study was to determine the prevalence of Staphylococcus aureus and risk factors for the acquisition of MRSA (Methicillin Resistant Staphylococcus aureus) as the main cause of skin and soft tissue infections. S. aureus were characterized for the presence of PVL, TSST-1 and mecA genes. SCCmec typing was carried out in mecA positive strains and PFGE was performed only in these strains. During the study period, 127 outpatients attending a dermatology clinical the Botucatu Medical School, a regional tertiary hospital in Botucatu, Sao Paulo, Brazil, were diagnosed with active skin infections. A total 66 (56.9%) S. aureus strains were isolated. The methicillin resistance gene mecA was detected in seven (10.6%) S. aureus strains. The SCCmec types... Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Resistance; Skin infections; Virulence; Epidemiology. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400034 Comparative analysis of agr groups and virulence genes among subclinical and clinical mastitis Staphylococcus aureus isolates from sheep flocks of the Northeast of Brazil BJM Almeida,Lara M. de; Almeida,Mayra Zilta P.R.B. de; Mendonça,Carla L. de; Mamizuka,Elsa M.. Staphylococcus aureus is one of the most frequent mastitis causative agents in small ruminants. The expression of most virulence genes of S. aureus is controlled by an accessory gene regulator (agr)locus. This study aimed to ascertain the prevalence of the different agr groups and to evaluate the occurrence of encoding genes for cytotoxin, adhesins and toxins with superantigen activity in S. aureus isolates from milk of ewes with clinical and subclinical mastitis in sheep flocks raised for meat production The agr groups I and II were identified in both cases of clinical and subclinical mastitis. Neither the arg groups III and IV nor negative agr were found. The presence of cflA gene was identified in 100% of the isolates. The frequency of hla and lukE-D... Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Agr groups; Virulence genes; Mastitis; Sheep flock. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200026 Comparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from bovine subclinical mastitis BJM Melo,Poliana de Castro; Ferreira,Luciano Menezes; Nader Filho,Antônio; Zafalon,Luiz Francisco; Vicente,Hinig Isa Godoy; Souza,Viviane de. Biofilm formation is considered to be a selective advantage for Staphylococcus aureus mastitis isolates by facilitating bacterial persistence in the udder. It requires attachment to mammary epithelium, proliferation and accumulation of cells in multilayers. The objective of this study was to determine the sensitivity and specificity of three techniques for the detection of S. aureus biofilm-positive strains. Two phenotypic tests, including growth on microtitre plates and Congo red agar, were compared with a PCR technique using 94 S. aureus strains obtained from cows with subclinical mastitis from two farms in the state of São Paulo. These strains were characterised by in vitro slime production on Congo red agar, biofilm formation on microtitre plates and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilms; Bovine mastitis; Phenotypic and molecular analysis; Staphylococcus aureus. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100017 Complete substitution of the Brazilian endemic clone by other methicillin-resistant Staphylococcus aureus lineages in two public hospitals in Rio de Janeiro, Brazil BJID Chamon,Raiane Cardoso; Ribeiro,Sthefanie da Silva; Costa,Thaina Miranda da; Nouér,Simone Aranha; Santos,Katia Regina Netto dos. Abstract Staphylococcus aureus is an important cause of bloodstream infections. Therefore, the main purpose of this work was to characterize a collection of 139 S. aureus isolates from bloodstream infections in two public hospitals in relation to their antimicrobial susceptibility profile, staphylococcal cassette chromosome mec types, and clonal relationship. Methicillin resistance and resistance to other 12 agents were accessed by the disk diffusion test. Minimum inhibitory concentration to mupirocin was also determined. The SCCmec types were accessed by multiplex PCR, and the clonal relationship was determined by pulsed field gel electrophoresis method and restriction modification system characterization. Besides, multilocus sequence typing was performed... Tipo: Info:eu-repo/semantics/other Palavras-chave: Staphylococcus aureus; Bloodstream infections; USA100; Mupirocin resistance. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000200185 Concentração mínima inibitória de dez antimicrobianos para amostras de Staphylococcus aureus isoladas de infecção intramamária bovina Arq. Bras. Med. Vet. Zootec. Brito,M.A.V.P.; Brito,J.R.F.; Silva,M.A.S.; Carmo,R.A.. Determinou-se a concentração mínima inibitória (CMI) de ampicilina, cefalotina, eritromicina, gentamicina, neomicina, norfloxacina, oxacilina, penicilina G, tetraciclina e tilosina para 112 amostras de Staphylococcus aureus isoladas de infecções intramamárias bovina, em 33 rebanhos leiteiros da Zona da Mata do Estado de Minas Gerais. Foram isoladas 24 amostras de infecção clínica, 66 de subclínica e 22 de infecção crônica. As amostras de infecção crônica foram isoladas repetidas vezes dos mesmos quartos mamários de nove vacas de um rebanho, no período de 13 meses. A CMI foi realizada em ágar Mueller Hinton, com concentrações entre 0,015 e 128µgml-1 de cada antimicrobiano. As amostras da American Type Culture Collection (ATCC) recomendadas para controle de... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mastite bovina; Staphylococcus aureus; Susceptibilidade antimicrobiana; Concentração mínima inibitória; Beta-lactamase. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000500003 Conjunctival microbial flora of clinically normal persons who work in a hospital environment BJM Trindade,Rita de Cássia; Bonfim,Ana Cristina Rocha; Resende,Maria Aparecida. The objective of the work was to study the microbiota of the conjunctival secretion of health professionals. Samples were collected from the clinically normal eyes of 40 health professionals in four different sectors, Proctology, General Intensive Care Unit (ICU), Male Ward, and Oncology of Hospital das Clínicas Dr. Augusto Leite, Aracaju, SE, Brazil. Ten professionals from each sector were selected. The samples were inoculated into various culture media: blood agar, Chapman agar, EMB medium (Teague), and Sabouraud dextrose agar with chloramphenicol. The bacteria most frequently isolated from all the sectors were Staphylococcus epidermidis (45.0%) and Bacillus sp (29.0%). The least frequent bacteria were Proteus sp (6.1%), Staphylococcus aureus (4.1%),... Tipo: Info:eu-repo/semantics/article Palavras-chave: Conjunctival microbiota; Hospital environment; Staphylococcus epidermidis; Staphylococcus aureus. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100004 Consórcio suíno-peixe: aspectos ambientais e qualidade do pescado R. Bras. Zootec. Pilarski,Fabiana; Tomazelli Júnior,Osmar; Casaca,Jorge de Matos; Garcia,Flávio Roberto Mello; Tomazelli,Ingrid Boesche; Santos,Ieda Rottava dos. Amostras de músculo de carpa comum (Cyprinus carpio) e de água de três viveiros fertilizados com dejetos de suínos e de um viveiro em que os peixes foram alimentados com ração comercial para peixes foram analisadas durante seis meses. O objetivo foi observar a presença de bactérias patogênicas, como Salmonella, Staphylococcus aureus e coliformes totais e fecais, em peixes comercializados in natura no município de Chapecó, Santa Catarina. Foram aferidos quinzenalmente, na água dos quatro viveiros, oxigênio dissolvido, pH, turbidez, alcalinidade total, dureza total, amônia, nitrato e ortofosfato, bem como a quantidade de coliformes totais e fecais. A análise microbiológica músculo dos peixes demonstrou ausência de Salmonella em todas as amostras. A contagem... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyprinus carpio; Dejetos suínos; Salmonella; Staphylococcus aureus; Coliformes totais e coliformes fecais. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982004000200001 Cost-effectiveness of linezolid versus vancomycin in mechanical ventilation-associated nosocomial pneumonia caused by methicillin-resistant staphylococcus aureus BJID Machado,Adão R. L.; Arns,Clovis da Cunha; Follador,Wilson; Guerra,Aline. Linezolid, an oxazolidinone-class antimicrobial agent, is a new drug; its use has frequently been questioned due to its high price. However, recent trials have demonstrated that the use of linezolid in mechanical ventilation-associated nosocomial pneumonia caused by methicillin-resistant Staphylococcus aureus (VAP-MRSA) may be justified due to its improved efficacy compared to vancomycin. Price and cost have different magnitudes, and clinical efficacy should always be considered in the decision-making process. Our objective was to determine whether linezolid treatment was more cost-effective than vancomycin for treating VAP-MRSA. METHODOLOGY: Elaboration of an economic model from a metanalysis of previous clinical trials comparing both drugs, through a... Tipo: Info:eu-repo/semantics/article Palavras-chave: Linezolid; Vancomycin; Staphylococcus aureus; Pneumonia; Ventilator; Cost; Pharmacoeconomic. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000300001 Cultura, crescimento e identificação de bactérias do gênero Staphylococcus aureus em leite de cabra. Infoteca-e CHAPAVAL, L.; AGUIAR, V. M. P.; SOUSA, A. P. B. de; MIRANDA, K. P. de; MORORÓ, A. M.; MAGALHÃES, D. C. T.. bitstream/CNPC-2010/23045/1/ct41.pdf Tipo: Circular Técnica (INFOTECA-E) Palavras-chave: Leite de cabra; Staphylococcus aureus; Bactéria; Microrganismo. Ano: 2009 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/748116 Daptomycin to bone and joint infections and prosthesis joint infections: a systematic review BJID Telles,João Paulo; Cieslinski,Juliette; Tuon,Felipe Francisco. ABSTRACT Backgroud: Daptomycin has been used in bone and joint infections (BJI) and prosthesis joint infections (PJI) considering spectrum of activity and biofilm penetration. However, the current experience is based on case reports, case series, cohorts, and international surveys. The aim of this systematic review was to evaluate studies about daptomycin treatment efficacy in BJI/PJI compared to other antibiotic regimens. Methods: PubMed, LILACS, Scielo and Web of Science databases were searched for articles about daptomycin and treatment of BJI and PJI from inception to March 2018. Inclusion criteria were any published researches that included patients with BJI treated with daptomycin. Diagnosis of BJI was based on clinical, laboratory and radiological... Tipo: Info:eu-repo/semantics/article Palavras-chave: Daptomycin; Staphylococcus aureus; Osteomyelitis; Joint infection; Arthritis. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000300191 DERMATITE ESTAFILOCÓCICA EM OVINO NO RIO GRANDE DO SUL Ciência Rural Ribeiro,Luiz Alberto Oliveira; Rodrigues,Norma Centeno; Fallavena,Luiz Cesar Bello. RESUMO Relata-se um caso de Dermatite Estafilocócica ocorrido em ovelha criada a campo no Rio Grande do Sul. São descritas e discutidas as lesões macroscópicas e microscópicas encontradas, assim como o isolamento do Staphylococcus aureus da lesão. Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Dermatite estafilocócica; Ovinos; Rio Grande do Sul. Ano: 1994 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781994000200035 Detecção da toxina-1 da síndrome do choque tóxico em amostras de Staphylococcus aureus isoladas de mastite bovina Arq. Bras. Med. Vet. Zootec. Cardoso,H.F.T.; Carmo,L.S.; Silva,N.. Este trabalho teve por objetivo caracterizar a produção da toxina da síndrome do choque tóxico (TSST-1) e de enterotoxinas estafilocócicas (SE) A, B, C e D em 127 amostras de S. aureus, isoladas de amostras de leite proveniente de vacas com mastite no Estado de Minas Gerais, entre 1994 e 1997. A verificação da produção de toxinas foi feita pela técnica de sensibilidade ótima em placa. Das 127 amostras testadas, 60 (47%) eram produtoras de TSST-1 e 54 (43%) produtoras de SE, 38 amostras produziram SED (30%), 24 SEB (19%), 8 SEC (6%) e 4 SEA (3%). Estes resultados trazem preocupações quanto à saúde pública pela alta prevalência de amostras de S. aureus produtoras de TSST-1 e de enterotoxinas em isolamentos a partir de leite de vacas com mastite. Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; TSST-1; Enterotoxina; Mastite. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000100002 Detecção dos genes de Staphylococcus aureus, enterotoxinas e de resistência à meticilina em leite Arq. Bras. Med. Vet. Zootec. Dias,N.L.; Silva,D.C.B.; Oliveira,D.C.B.S.; Fonseca Junior,A.A.; Sales,M.L.; Silva,N.. Realizou-se a detecção do gene de Staphylococcus aureus, de enterotoxinas e de resistência à meticilina com extração de DNA feita diretamente de amostras de leite. Das 200 amostras estudadas, 145 (72,5%) amplificaram o gene femA, e estas foram analisadas quanto à presença dos genes sea, seb, sec e mecA. Os genes das enterotoxinas mais prevalentes foram: sea (60%), seb (37,9%) e sec (6,9%). Foram encontradas 18 amostras de leite (11,0 %) com S. aureus portadores do gene mecA. A detecção de S. aureus diretamente do leite, sem a necessidade de isolamento bacteriano e a caracterização do potencial enterotoxigênico, demonstra que a técnica de PCR é muito útil para estudos epidemiológicos das infecções estafilocócicas da glândula mamária. O alto percentual... Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Enterotoxinas; MRSA; PCR. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000600036 Detection by multiplex PCR of Staphylococcus aureus , S. intermedius and S. hyicus in artificially contaminated milk Ciência Rural Gandra,Eliezer Avila; Fernandez,Maria Aparecida; Silva,Jorge Adolfo; Silva,Wladimir Padilha da. ABSTRACT: This research aimed to detect coagulase-positive Staphylococcus (CPS) directly in samples of artificially contaminated milk, using multiplex PCR (mPCR). Standard and isolated bacterial strains of S. aureus, S. epidermidis, S. hyicus, S. intermedius, Listeria monocytogenes and Escherichia coli species were used, evaluating the specificity and detection limit of mPCR, for artificially contaminated UHT milk. Primers specific for the nuc gene (NUC1-NUC2 were used for S. aureus, NUC3-NUC4 for S. hyicus and NUC5-NUC6 for S. intermedius). It was possible to detect the three target species by mPCR, directly from bovine whole milk, with adequate specificity and acceptable detention limit for identification of coagulase-positive Staphylococcus (CPS) in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Staphylococcus hyicus; Staphylococcus intermedius; Multiplex PCR; Nuc gene. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000801418 Detection of different Staphylococcus aureus strains in bovine milk from subclinical mastitis using PCR and routine techniques BJM Vieira-da-Motta,Olney; Folly,Márcio Manhães; Sakyiama,Cássia Camargo Hagen. Contamination of fresh milk with Staphylococcus aureus was assessed comparatively through routine phenotypic (coagulase tube test and coagulase slide test) and genotypic (PCR) screening of 128 S. aureus strains isolated from 555 milk samples. These samples were collected from 362 cows with subclinical mastitis, hosted in different dairy herds at various locations of the Northern and Northeastern rural areas of the State of Rio de Janeiro, 39.7% of which were CMT-positive. All S. aureus isolates tested positive for the presence of the coagulase gene by PCR and the isolates could be grouped into four distinct classes according to the size of the PCR product. The strains also yielded variable results when assayed with coagulase test. Taken together, these... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine mastitis; Staphylococcus aureus; Coagulase; PCR. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100007 Detection of methicillin resistance and slime factor production of Staphylococcus aureus in bovine mastitis BJM Ciftci,Alper; Findik,Arzu; Onuk,Ertan Emek; Savasan,Serap. This study aimed to detect methicillin resistant and slime producing Staphylococcus aureus in cases of bovine mastitis. A triplex PCR was optimized targetting 16S rRNA, nuc and mecA genes for detection of Staphylococcus species, S. aureus and methicillin resistance, respectively. Furthermore, for detection of slime producing strains, a PCR assay targetting icaA and icaD genes was performed. In this study, 59 strains were detected as S. aureus by both conventional tests and PCR, and 13 of them were found to be methicillin resistant and 4 (30.7%) were positive for mecA gene. Although 22 of 59 (37.2%) S. aureus isolates were slime-producing in Congo Red Agar, in PCR analysis only 15 were positive for both icaA and icaD genes. Sixteen and 38 out of 59 strains... Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; IcaA; IcaD; Slime; Mastitis. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200009 Detection of Staphylococcus aureus with an intermediate profile to vancomycin (VISA) isolate from Santa Maria, RS BJM Marques,Jaciane Baggiotto; Dalmolin,Tanise Vendruscolo; Bonez,Pauline Cordenonsi; Agertt,Vanessa Albertina; Campos,Marli Matiko Anraku de; Santos,Roberto Christ Vianna. Twenty-three isolates of Staphylococcus aureus resistant to methicillin have been analyzed, being found a clinical isolate of VISA through microdilution technique. The others techniques were unable to detect such isolates. This is the first study that shows the presence of VISA in clinical isolates in the city of Santa Maria-RS. Tipo: Info:eu-repo/semantics/other Palavras-chave: Staphylococcus aureus; Vancomycin; Microdilution. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100040 Detection of the mecA gene and identification of Staphylococcus directly from blood culture bottles by multiplex polymerase chain reaction BJID Rocchetti,Taisa Trevizani; Martins,Katheryne Benini; Martins,Patricia Yoshida Faccioli; Oliveira,Rogério Antonio de; Mondelli,Alessandro Lia; Fortaleza,Carlos Magno Castelo Branco; Cunha,Maria de Lourdes Ribeiro de Souza da. ABSTRACT Introduction: Staphylococcus spp. – both S. aureus, including methicillin-resistant strains (MRSA) and coagulase negative staphylococci (CoNS) – are relevant agents of healthcare-associated infections. Therefore, the rapid recognition of MRSA and methicillin-resistant CoNS from blood stream infections is critically important for patient management. It is worth noting that inappropriate empiric therapy has been associated with higher in-hospital mortality. Material and methods: In this study we evaluated a multiplex polymerase chain reaction (multiplex PCR) standardized to detect Staphylococcus spp., S. aureus, and mecA gene-encoded oxacillin resistance directly from blood culture bottles. A total of 371 blood cultures with Gram-positive... Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus spp.; Staphylococcus aureus; MRSA; MecA gene; Blood cultures; Multiplex PCR. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000200099 Registros recuperados: 148 Primeira ... 1 2 3 4 5 6 7 8 ... Última

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